Please click on below links to download the course file: 1-What is HPLC? 2-Basic terms and concepts. 3-HPLC instrumentation and trends. 4-Introduction to chromatography software. 5-Types of HPLC; isocratic & gradient. 6-Qualitative and quantitative determination. 7-Normal & reverse phase chromatography modes. 8-HPLC column and trends. 9-Column selection in reverse phase chromatography. 10-HPLC method development. 11-Regulatory[…]
Immunoprecipitation assay It is the technique of precipitating a protein “antigen” out of solution using “an antibody” that specifically binds to that particular protein “antigen” in a complex This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins Immunoprecipitation requires that the antibody be[…]
Electrophoresis is the motion of dispersed particles in a fluid (water) under the influence of constant electric field. Consist of: gel, samples, buffer (to supply ions) and electrical field (cathode & anode & battery & wires). Cathode (-) reduction takes place and Anode (+) oxidation take place Technique requires a dye for coloring the sample. Usually used in[…]
Abstract: Objective: The menstrual cycle represents a continuous state of change in terms of female sex steroid environment which are believed to affect fat storage and distribution in women. Progesterone is linked to increased fat storage while estrogen exerts anti- lipogenic effects. In this study, we investigate variations in the potent lipogenic factor, the acylation[…]