The SOCS2 Ubiquitin Ligase Complex Regulates Growth Hormone Receptor Levels

Immunoprecipitation assay

  • It is the technique of precipitating a protein “antigen” out of solution using “an antibody” that specifically binds to that particular protein antigen” in a complex
  • This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins
  • Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure. (Agarose solid-phase support )
  • The solution used in the form of a crude lysate of an animal tissue

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