Immunoprecipitation assay
- It is the technique of precipitating a protein “antigen” out of solution using “an antibody” that specifically binds to that particular protein “antigen” in a complex
- This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins
- Immunoprecipitation requires that the antibody be coupled to a solid substrate at some point in the procedure. (Agarose solid-phase support )
- The solution used in the form of a crude lysate of an animal tissue