Objectives: Define clinical biochemistry. Describe the uses of biochemical tests. Describe the routine tests done in clinical chemistry. Describe sample analysis and interpretation of results. Highlight the importance of routine clinical chemistry tests in the diagnosis, prognosis, monitoring and screening for disease. Describe special chemistry test and correlate lab results with disease states. Basic Principles[…]
Hamed Al-Riyami, Mohsin Al-Saleh and Jumana Saleh Biochemistry department, Faculty of Medicine, Sultan Qaboos University, Muscat, Oman It is well established that hyperlipidemia is associated with formation of smaller, denser LDL particles (sdLDL) in plasma. Many studies suggested that sdLDL particles have increased atherogenicity compared to larger buoyant subtypes. Several reasons were suggested in this[…]
Oxidation of low density lipoproteins (LDL) is a modification believed to be a key event in the development of atherosclerosis. Oxidized LDL (oxLDL) particles are recognized by macrophage scavenger receptors where macrophages become loaded with cholesterol converting into “foam cells” that accumulate into fatty streaks initiating atherosclerosis events. In vitro studies suggest that small LDL[…]
NIDDM is a significantly public health problem in the Sultanate of Oman. Based on 1991 regional DM survey in Omani population of age above 20 years indicate that 10.5% are DM, and about 10.5% are IGT. Only one-third of diabetic subjects knew that they had diabetes. The survey found that diabetes was more common in[…]
Please click on below links to download the course file: 1-What is HPLC? 2-Basic terms and concepts. 3-HPLC instrumentation and trends. 4-Introduction to chromatography software. 5-Types of HPLC; isocratic & gradient. 6-Qualitative and quantitative determination. 7-Normal & reverse phase chromatography modes. 8-HPLC column and trends. 9-Column selection in reverse phase chromatography. 10-HPLC method development. 11-Regulatory[…]
Immunoprecipitation assay It is the technique of precipitating a protein “antigen” out of solution using “an antibody” that specifically binds to that particular protein “antigen” in a complex This process can be used to isolate and concentrate a particular protein from a sample containing many thousands of different proteins Immunoprecipitation requires that the antibody be[…]
Electrophoresis is the motion of dispersed particles in a fluid (water) under the influence of constant electric field. Consist of: gel, samples, buffer (to supply ions) and electrical field (cathode & anode & battery & wires). Cathode (-) reduction takes place and Anode (+) oxidation take place Technique requires a dye for coloring the sample. Usually used in[…]
Abstract: Objective: The menstrual cycle represents a continuous state of change in terms of female sex steroid environment which are believed to affect fat storage and distribution in women. Progesterone is linked to increased fat storage while estrogen exerts anti- lipogenic effects. In this study, we investigate variations in the potent lipogenic factor, the acylation[…]